Effects of MACC1 silencing on proliferation, apoptosis, and invasion of human ovarian cancer drug-resistant cell line

DENG Youxing; SHI Huirong; LI Xia; ZHANG Ruitao

doi:10.3969/j.issn.1002-266X.2017.26.006

Abstract：Objective To observe the proliferation, apoptosis, and invasion changes of human ovarian cancer drug-resistant cell line after metastasis-associated in colon cancer-1 (MACC1) silencing by using gene silencing technique and its possible mechanism.Methods The human ovarian cancer drug-resistant SKOV-3/DDP cells were divided into the empty plasmid group and the transfection group, which were transfected with p-super-EGFP and p-super-EGFP-MACC1 shRNA.In addition, the untreated SKOV-3/DDP cells were taken as the control group.The expression of MACC1 mRNA was detected by RT-PCR,Western blotting was used to detect the expression of MACC1 protein, MTT assay was used to detect cell proliferation, flow cytometry was used to detect the apoptosis, the adhesion test was used to detect the adhesion of cells in vitro, Transwell chamber was used to detect cell invasion, and the protein expression of C-met, ERK1/2 and p-ERK1/2 was detected by Western blotting.Results Compared with the blank control group and empty plasmid group, the MACC1 mRNA and protein levels were lower, the proliferation ability decreased at 48 and 72 h, apoptosis rate increased, the adhesion and invasion abilities increased, the expression of C-met, p-ERK1/2, MMP-2, MMP-9, and Caspase-3 decreased, and the expression of cleaved caspase-3 protein increased in the transfection group (all P<0.05).There was no statistically significant difference between the blank control group and empty plasmid group (all P>0.05).Conclusion Silencing MACC1 gene inhibits the proliferation, adhesion, and invasion abilities of SKOV-3/DDP cells, and promotes apoptosis, which may be related to the inhibition of HGF/C-met and ERK1/2 pathway.