Effects of peimine on cell viability and apoptosis of multidrug resistant human leukemia cells
QI Yan
LIAO Bin
XU Chengbo
HUANGFU Zhenping
CHEN Jiawei
Abstract：Objective To investigate the effects of peimine on cell viability and apoptosis of multidrug resistant human leukemia K562/A02 cells and the function of reactive oxygen species (ROS) in this progress.Methods The K562/A02 cells were divided into three groups.Cells in the drug group were treated with peimine with the final concentrations of 100, 200, and 400 μmol/L, respectively.Cells in the N acetyl cysteine (NAC) pretreatment group were treated with 5 mmol/L antioxidant NAC, followed by 400μmol/L peimine.Cells in the control group were treated with an equal volume of dissolution medium RPMI1640.The cell viability of each group was detected by MTT, the apoptosis was detected by flow cytometry, and the levels of ROS and glutathione (GSH) in the cells were detected by UV spectrophotometry.Results Compared with the control group, the cell viability of the drug group decreased (P<0.05 or P<0.01), intracellular ROS level increased (P<0.01), GSH concentration decreased (P<0.01), and the apoptosis rate increased (P<0.05).However, the cell viability, the apoptosis rate, and the ROS level in NAC pretreatment group showed no obvious change (all P>0.05).Conclusion Peimine inhibits the cell viability and induces apoptosis of multidrug resistant leukemia K562/A02 cells through inducing the ROS outbreak and thus increasing the GSH content.
Keywords：peiminechronic myeloid leukemiaK562/A02multidrug resistancereactive oxygen speciescell viabilityapoptosis
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 17-20 )