Effect of FGF-21 on glucose uptake of insulin-resistant liver cells
BAI Jie
WANG Jinxin
SONG Zixuan
WU Zhimin
GAO Yan
ZHU Ze
LI Guangming
Abstract：Objective To observe the effect of fibroblast growth factors-21 (FGF21) on glucose uptake of insulin-resistant (IR) liver cells.Methods The fluorescently labeled FGF21 mRNA with certain stability was synthesized in vitro.The liver cells were cultured for 72 h in RPMI-1640 medium supplemented with 34.4 μmol/L recombinant insulin and 1 μmol/L dexamethasone to establish IR cell models, and then IR cell models were divided into the model control group, insulin group, and FGF21 group.We did not add any drugs into the model control group.The insulin group was treated with 1 μmol/L recombinant insulin and FGF21 group was transfected with FGF21 mRNA.The cell glucose uptake in the three groups was measured by glucose oxidase (GOD-POD) assay.The mRNA and protein expression of glucose transporter 1 (GLUT1) was detected by real-time fluorescent quantitative PCR and Western blotting.Results Compared with the model control group, the glucose uptake of the FGF21 group increased and the expression of GLUT1 mRNA and protein increased (all P<0.05).Compared with the model control group, the glucose uptake of the FGF21 group did not change significantly in the insulin group, and no significant difference was found in the GLUT1 mRNA and protein expression as compared with that of the model control group (all P>0.05).Conclusion FGF21 mRNA synthesized in vitro can improve the glucose uptake of IR liver cells, and its mechanism is related to the increase of GLUT1 expression.
Keywords：fibroblast growth factors-21glucose transporter 1in vitro transcription of RNAglucose uptakeinsulin resistancehepatic cells
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 5-8 )