Effects of miR-219-5p on proliferation,apoptosis,and invasion of pancreatic carcinoma cells

LI Liping; XU Dan; YE Jun; WU Shun; MA Songlin

doi:10.3969/j.issn.1002-266X.2017.25.004

Abstract：Objective To investigate the effects of miRNA-219-5p on the proliferation,apoptosis,and invasion of pancreatic carcinoma cells and its mechanism. Methods Real-time fluorescence quantitative PCR (qPCR)was carried out to determine miR-219-5p expression in the pancreatic carcinoma cell lines Panc-1,AsPC-1,and Bxpc-3 and normal pancreatic ductal epithelial cell line HPDE6-C7. Further,Panc-1 cells were chosen and were divided into two groups:the normal control group (NC group)(transfection with miR-219-5p scramble)and miR-219-5p group (transfection with miR-219-5p mimics). The cell proliferation and apoptosis were detected by MTT assay and flow cytometry. The invasion ability was checked by transwell assay. The expression of epidermal growth factor receptor (EGFR)and Cleaved Caspase-3 pro-teins was examined by Western blotting. Results MiR-219-5p expression was lower in pancreatic carcinoma cell lines Panc-1,AsPC-1,and Bxpc-3,as compared with that in the pancreatic ductal epithelial cell line HPDE6-C7 (all P <0. 01). The OD490nm of the miR-219-5p group was significantly lower than that of the NC group at 72,96,and 120 h (all P < 0. 01);the apoptosis rate was higher (all P < 0. 01). The invasive cells were (75. 3 ± 6. 9)/ Hp in the miR-219-5p group,which was significantly lower than that [(172. 5 ± 10. 5)/ Hp]in the NC group (P < 0. 01). The expressive level of EGFR in the miR-219-5p group was significantly lower than NC group (0. 31 ± 0. 09 vs. 1. 0,P < 0. 01). The expres-sion of Cleaved Caspase-3 was up-regulated in the miR-219-5p group as compared with that of NC group (2. 7 ± 0. 15 vs.1. 0,P < 0. 01). Conclusions The miR-219-5p is lowly expressed in pancreatic carcinoma cell lines. The over-expression of miR-219-5p can inhibit the proliferation and invasion of pancreatic carcinoma cells,and induce the apoptosis,possibly though the down-regulated protein expression of EGFR and up-regulated protein expression of Cleaved Caspase-3.