Effect of repeated limb remote ischemia conditioning on spatial memory and learning in chronic cerebral hypoperfusion rats
ZHANG Anbo
LI Ning
HAN Rongrong
REN Changhong
Abstract：Objective To explore the effect and mechanism of repeated limb remote ischemic conditioning (LRIC) on spatial memory and learning in chronic cerebral hypoperfusion rats.Methods Sixty male SD rats were selected and randomly divided into the sham group, model group, single LRIC group, and multiple LRIC group.The rats in the latter three groups underwent bilateral carotid artery occlusion (2VO) to establish chronic cerebral hypoperfusion models, and the bilateral carotid arteries of rats in the control group were only separated without ligation.Three days after surgery, rats in the single LRIC group received LRIC just once, the detailed method was that ribbon gauzes were used to ligature hind limbs for occluding the femoral artery, ligating for 10 min, loosening for 10 min, and three circles each time.Rats in the multiple LRIC group received LRIC once a day for 6 w.Four weeks after LRIC was finished, Morris water maze test was used to test escape latency and target quadrant percentage, cerebral blood flow of hippocampal CA1 region was measured with laser Doppler, and the ultrastructure of axons and blood vessels in hippocampal CA1 region was observed by electron microscopic.Results Escape latency of the sham group, multiple LRIC group was shorter than that of the model group and single LRIC group, but the target quadrant percentage was higher than that of the model group and single LRIC group (P<0.05 or P<0.01).The relative cerebral blood flow of hippocampal CA1 region in the sham group, model group, single LRIC group, and multiple LRIC group was 100%, 61.3%±11.7%, 59.5%±13.1%, and 80.2%±14.6%, respectively.The relative cerebral blood flow of the sham group and multiple LRIC group was higher than that of the model group and single LRIC group (all P<0.05).Endothelium was not smooth;thickening of the basement membrane, obvious peripheral edema, axonal fracture, and plate separation were observed in CA1 region of the model group;the changes mentioned above alleviated significantly in the multiple LRIC group (blood vessels and axons changes were not tested because there was no significant difference in spatial learning memory and cerebral blood flow between the single LRIC group and model group).Conclusion Repeated LRIC can improve cognitive impairment caused by chronic cerebral blood flow hypoperfusion by increasing blood supply in hippocampus and inhibiting microvascular injury in hippocampus.
Keywords：chronic cerebral hypoperfusionlimb remote ischemic conditioningMorris water maze testrats
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 16-19,后插1 )