Inhibitory effect of total flavonoids of lichi on proliferation of hepatic stellate cells
CHEN Guihong
LIU Rui
LIU Shengnan
LUO Weisheng
Abstract：Objective To explore the inhibitory effect of total flavonoids of lichi (TFL) on proliferation of rat hepatic stellate cells (HSC-T6) and to study the mechanism.Methods The cultured rat hepatic stellate cells in vitro were randomly divided into four groups as follows: the control group, low-dose, medium-dose, and high-dose TFL groups.Each group was cultured with cell culture medium (10%FBS included).TFL with final concentrations of 160, 320, and 640 μg/mL were added into the low-dose, medium-dose, and high-dose TFL groups.MTT method was used to detect the cell proliferation (A value) at 24, 48 and 72 h after treatment.After acting for 72 h, the mRNA and protein expression of matrix metalloproteinase 2 (MMP-2) and tissue inhibitor of metalloproteinase 1 (TIMP1) in rat hepatic stellate cells was detected by RT-PCR and Western blotting, respectively.Results As time went on, the A value of each group significantly decreased except the high-dose TFL group after exposure to TFL for 48 and 72 h (all P<0.05).After exposure for 24 h, the A value of HSC-T6 cells in each group had no obvious change;after exposure for 48 h, the A value of HSC-T6 cells in the low-dose, medium-dose, and high-dose TFL groups were lower than that of the control group, and the high-dose TFL group was the most significant (all P<0.05).After exposure for 72 h, the A value of HSC-T6 cells decreased with the increasing concentrations of TFL (all P<0.05).After 72 h, the mRNA and protein expression of MMP-2 and TIMP1 of the low-dose, medium-dose, and high-dose TFL groups was lower that that of the control group, and with the increase of drug concentration, the expression decreased gradually (all P<0.05).Conclusion TFL can inhibit the proliferation of HSC-T6 cells with a time-and dose-dependent manner, and the inhibition may be related to the down-regulation of MMP-2 and TIMP1 expression.
Keywords：hepatic fibrosistotal flavonoids of lichihepatic stellate cellsmatrix metalloproteinase 2tissue inhibitor of metalloproteinase 1
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 9-12 )