Influence of Yupingfeng powder on proportion and function of lung-resident innate lymphoid cells in mice under hypothermia
AN Lei
GUO Yuqi
WANG Li
SUN Xiaoyan
LYV Yanmin
LAN Hongyun
YAO Chengfang
Abstract：Objective To observe the influence of Yupingfeng powder on the proportion and function of lung-resident innate lymphoid cell subsets (ILCs) in mice under hypothermia.Methods Twenty-four C57BL/6 mice were randomly divided into three groups:the control group,the hypothermia model group,and the group of Yupingfeng powder (YPF group).The mice in the control group were not given any treatment.The mice from hypothermia model group and YPF group were both exposed to a cold condition (10℃ ±1℃) in a cryogenic vessel,3 h/d,for 3 days.The mice of the YPF group were given YPF on day 7 before cold exposure until the end of the experiment.ILCs,and its three subsets (ILCI,ILC2,and ILC3) in lung lymphocytes were detected by flow cytometry.The expression of IFNγ,IL-13,and IL-17A was detected by RT-PCR.Results Compared with the control group,the proportions of ILCs,ILC1,ILC2,and ILC3 were lower in the hypothermia model group (all P ＜ 0.05) as well as the mRNA expression of IFFNγ,IL-13,and IL-17A (all P ＜0.01).Compared with the hypothermia model group,the proportions of ILCs,ILC1,ILC2,and ILC3 were higher in the YPF group (all P＜0.05),as well as the mRNA expression of IFNγ,IL-13,and IL-17A (all P ＜ 0.05).Conclusions Yupingfeng powder may reverse the inhibitory effect of hypothermia on ILCs and the functional factors in lung tissues.
Keywords：hypothermiainnate lymphoid cellslungYupingfeng powdertraditional Chinese drugs
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 12-14 )