Effect of ex vivo oxygenated blood continuous perfusion on pulmonary function in pigs after lung transplantation
HUANG Ailan
WEI Huijun
LONG Xiaomao
HU Yanyan
JIANG Xiaohuan
Abstract：Objective To observe the effect of ex vivo oxygenated blood continuous perfusion on lung function in pigs after lung transplantation.Methods Twenty-four Guangxi miniature pigs were randomly divided into the observation group and the control group,and each group had 6 donors and 6 receptors.After being completely resected,bilateral lungs of the donors were preserved by ex vivo oxygenated blood continuous perfusion and static cold storage for eight hours,respectively,and then the left lungs were transplanted into the receptors.We compared the PaO2/FiO2 of the left lower pulmonary vein between the observation group and the control group at 0.5,2,4,6 h after reperfusion.Using HE staining to observe the pulmonary tissues,which were collected at the time of before preserving (T0),after eight hours saved (T1),three (T2) and six (T3) hours after reperfusion.Results The PaO2/FiO2 of the left lower pulmonary vein were (482.50 ± 30.72),(467.17 ±31.06),(441.17 ± 17.86),and (422.33 ±46.45) mmHg in the observation group,versus (413.83 ± 18.53),(389.67 ± 7.28),(313.67 ± 24.53),and (308.50 ± 50.45) mmHg in the control group,at 0.5,2,4,6 h after reperfusion,respectively.There were significant differences between 6 h and 0.5 h in observation group,between 4 h,6 h and 0.5 h in the control group as well as between these two groups at each time point (all P ＜ 0.05).Pathological examination showed that,as the time went by,alveolar interval became wider gradually,alveolar structure gradually collapsed,alveolar edema got obvious,and exudation was seen in the alveolar cavity.The organization structure of pulmonary tissue in the control group changed worse than that in the observation group,and the pulmonary edema grading of the control group was higher than that of the observation group at T3 (P ＜ 0.05).Conclusion The donor lungs after being protected with ex vivo oxygenated blood continuous perfusion can obtain a better oxygenation and maintain a relatively complete structure of lung tissues.
Keywords：lung transplantationdonor preservationoxygenated bloodex vivo continuous lung perfusion storagecold static storageischemia-reperfusion injurylung functionanimal experiment
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 5-8 )