Construction of stable cervical cancer CasKi cell line with low expression of SND1 and its biological function changes
MENG Hao
FU Xiao
ZHANG Chunyan
XIN Lingbiao
REN Yuanyuan
YANG Jie
HE Jinyan
Abstract：Objective To construct a stable cervical cancer CasKi cell line with low expression of SND1 and detect the effect of SND1 on the biological function of cervical cancer cells. Methods CasKi cells were divided into six groups: the blank control group, negative control group, and interference groups 1-4. The cells in the negative control group and four interference groups were transfected with TRC2-pLKO-puro Vector empty lentivirus and SND1 shRNA(1-4). The stable CasKi cells were screened out by puromycin. Western blotting and RT-PCR were used to detect the expression of SND1 protein and mRNA to confirm the transfection efficiency. The proliferation of CasKi cells was detected by CCK-8 (OD450). The migration distance of CasKi cells was detected by cell Scratch test.Results Cells in the blank control group all die, and some in the negative control group and four interference groups survived, which indicated the stable cells were successfully constructed. Compared with the blank group and negative control group, the mRNA and protein expression levels of SND1 gene decreased in the four interference groups, and significant difference was found between the interference groups 3 and 4 (all P<0.05). Significant difference was found in the OD450 and migration between the blank group, negative control group, and the interference groups 3 and 4 (all P<0.05).Conclusion The stable CasKi cell line with low expression of SND1 is successfully established, and its proliferation and migration abilities decrease.
Keywords：cervical neoplasmsSND1 proteinCasKi cellscell proliferationcell migration
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1-4 )