Effect of retigabine on contraction of smooth muscle of rat duodenum in vitro
GAO Di
CHEN Yuanjin
LIU Yudan
ZHANG Nan
SHA Lei
Abstract：Objective To investigate the effect of retigabine on contraction of the smooth muscle of rat duodenum and to discuss its mechanism.Methods The rat duodenum was cut into segments with a length of about 1.5 cm.We used BL-420F Biological Function Experiment System for tension recording.After tension curve was stabilized, we performed the following experiments and each experiment was repeated 6 times.① We added 3.125, 6.25, 12.5, 25, 50, 100, 150, 200 μmol/L of retigabine into the solution.The recording time of each concentration was 15 min and each concentration was rinsed with Kreb's solution for 20 min.The mean amplitude of the smooth muscle tension of duodenum in vitro was measured before administration and after treatment with different concentrations of retigabine.② After 10 μmol/L XE-991 was added for 15 min, we added 20 μmol/L retigabine for 15 min.Then we compared the percent of mean amplitude of the smooth muscle tension of duodenum in vitro before and after retigabine.③ After 20 μmol/L retigabine was added for 15 min, we added 10 μmol/L XE-991 for 15 min.Then we compared the percent of mean amplitude of the smooth muscle tension of duodenum in vitro before and after XE-991.Results ① The percent of mean amplitude of the smooth muscle tension of duodenum in vitro before retigabine intervention was 100%.The percents of mean amplitude which were treated with retigabine were lower than that of the control group (0 μmol/L), P<0.05.In the range of 6.25-200 μmol/L, the percent of mean amplitude decreased gradually with the increase of retigabine concentration, P<0.05.② The percent of mean amplitude of smooth muscle tension which was incubated by XE-991 did not change significantly before and after retigabine intervention, P>0.05.③ The percent of mean amplitude of smooth muscle tension which was incubated by retigabine was higher than that after being given XE-991, P<0.05.Conclusion In the range of 6.25-200 μmol/L, retigabine can inhibit the contraction of rat duodenal smooth muscle in a dose-dependent manner, and this inhibitory effect may be due to the activation of potassium channel.
Keywords：retigabineduodenumsmooth musclecontractionpotassium channelin vitro experiment
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 17-19 )