Effect of NOX pathway in oxidative stress-induced apoptosis of cervical cancer cells
WANG Jing
WANG Hongtao
MA Hua
SUN Meiqun
ZHAO Weidong
Abstract：Objective To investigate the oxidative stress status of different types of cervical cancer cells, and the role of nicotinamide adenine dinucleotide phosphate oxidase (NOX) pathway in oxidative stress-induced apoptosis of cervical cancer cells.Methods Flow cytometry (FCM) was employed to detect the levels of total reactive oxygen species (ROS) of different types of cervical cancer cells and intracellular ROS levels derived from mitochondrial and NOX pathway before and after treatment with a NOX inhibitor, DPI.After DPI pretreatment and H2O2 action, flow cytometry was used to detect the apoptosis rate of different types of cervical cancer cells.Results HPV-positive cells showed higher levels of ROS as compared with HPV-negative cells (P<0.05).No significant difference was found in the mitochondrial pathway-derived ROS level between HPV-negative cells and HPV-positive cells (P>0.05).NOX inhibitor DPI significantly reduced ROS level in HPV-positive cells as compared with that before treatment (P<0.05).Compared with the control group and the HPV-negative cells, the apoptosis rate of HPV-positive cells increased after H2O2 induction and was higher than that of HPV-positive cells group with DPI pretreatment and with DPI pretreatment + H2O2 induction.Conclusion NOX pathway plays an important role in the process of HPV inducing cervical cancer, and pro-oxidant treatment may be more effective in killing HPV-positive cervical cancer cells.
Keywords：cervical carcinomahuman papilloma virusstressreactive oxygen speciesnicotinamide adenine dinucleotide phosphate oxidase
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 13-16 )