Growth of primary breast cancer cells cultured by Matrigel three-dimensional cell culture method and its sensitivity to adriamycin
ZHANG Zhen
QI Xiaomin
ZHANG Wei
ZHANG Pei
CAO Xuchen
XIAO Chunhua
Abstract：Objective To observe the growth of primary breast cancer cells cultured in Matrigel three-dimensional cell culture method and its sensitivity to adriamycin.Methods The primary breast cancer cells were cultured by two-dimensional monolayer culture and Matrigel three-dimensional cell culture methods.On the 1st, 3rd, 5th and 7th day of culture, Olympus IX70 inverted microscope was used to observe the morphology of primary breast cancer cells under the condition of two-dimensional monolayer culture and three-dimensional culture.CellTiter-Glo(R) Luminescent Cell Viability Assay was applied to observe the cell proliferation and its sensitivity to adriamycin.Primary breast cancer cell growth curve and drug sensitivity curve were drawn based on the observation results.Results The primary breast cancer cells with two-dimensional monolayer culture were in adherent growth, and the cancer cells gathered like a nest, with a variety of forms.The primary breast cancer cells with three-dimensional cell culture gathered into close multi-tumor cell microspheres.The proliferation of primary breast cancer cells cultured by two-dimensional monolayer culture on the 1st, 3rd, 5th and 7th day was 1.00±0.00, 1.39±0.29, 2.08±0.25 and 3.26±0.41.The proliferation of primary breast cancer cells cultured by Matrigel three-dimensional cell culture was 1.00±0.00, 1.25±0.08, 1.79±0.10 and 1.58±0.02.With the increase of adriamycin concentration, the inhibition rate of proliferation of primary breast cancer cells increased under two-dimensional monolayer culture and three-dimensional culture.But the proliferation inhibition rate of primary breast cancer cells with three-dimensional cell culture was lower than that with two-dimensional cell culture.Conclusion The primary breast cancer cells with Matrigel three-dimensional cell culture can gather into close spheres, the cell proliferation rate slows down, and the sensitivity of adriamycin decreases.Matrigel three-dimensional cell culture is better for culturing primary breast cancer cells.
Keywords：breast neoplasmsbreast carcinamaMatrigel three-dimensional cell culture methodcell morphologycell proliferationdrug sensitivity
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 17-20 )