Therapeutic effect of Shenqi Qiangjing capsule on spermatogenic function injury in rats by gavage
LIU Qian
TIAN Yuanchun
BIN Bin
LU Haiwang
Abstract：Objective To observe the therapeutic effect of Shenqi Qiangjing capsule on spermatogenic function injury in rats by gavage, and to explore its possible mechanism.Methods Forty SPF male rats were assigned into four groups: groups A, B, C, and the control group, with 10 rats in each group.Rats in the group A were given 800 mg/kg ornidazole and 1 600 mg/kg Shenqi Qiangjing capsule, rats in the group B were given 800 mg/kg ornidazole and 1 000 mg/kg Shengjing capsule, rats in the group C were given 800 mg/kg ornidazole to make the models with spermatogenic function injury, and rats in the control group were given the same volume of normal saline.All groups were fed by intragastric administration once a day for continuous 28 days.Then, we observed the growth of rats′ weight in each group, calculated the increase rate of weight, calculated the testis coefficient, tested the sperm concentration and motility rate in rats′ epididymis, and detected the expression of Bcl-2 and Bax protein in rats′ testes.Results On day one after the administration, the testis coefficient of group C was lower than that of the control group (P<0.05).The testis coefficients of group A and group B were higher than that of group C (both P<0.05).On day one after the administration, the sperm concentrations and the sperm motility rates of group A, group B and group C were significantly lower than that of the control group (all P<0.05);the sperm concentrations and the sperm motility rates of group A and group B were significantly higher than that of group C (all P<0.05).The Bcl-2 average optical densities of group A and group B were higher but the Bax average optical densities were lower than that of group C (all P<0.05).Conclusions Shenqi Qiangjing capsule has a good therapeutic effect on spermatogenic function injury in rats.Its mechanism may be related to inhibiting the expression of Bax protein, promoting the expression of Bcl-2 protein and thus inhibiting the apoptosis of spermatogenic cells in testes.
Keywords：Shenqi Qiangjing capsulespermatogenic function injurysperm motility rateB-cell lymphomaapoptosis-related gene
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 13-16 )