Expression of Vimentin and E-cadherin in human cervical carcinoma cell lines Hela and Siha transfected by FMNL2 gene siRNA
TAO Mingzhu
ZHANG Qiang
ZHOU Tiejun
YANG Chengwan
Abstract：Objective To investigate the expression of Vimentin and E-cadherin in human cervical carcinoma cell lines Hela and Siha transfected with Formin-like protein 2(FMNL2)gene small interference RNA (siRNA).Methods Both Hela cells and Siha cells were divided into the experimental group and control group, respectively.Cells in the experimental group were treated with 2 mL serum-free culture medium containing 100 nmol/L FMNL2-siRNA encapsulated by Lipofectamine 2000TM.Cells in the control group were treated with 2 mL serum-free culture medium.We used RT-PCR to detect the expression of FMNL2, Vimentin and E-cadherin mRNA in each group 72 h after transfection.Cellular immunohistochemistry was applied to detect the expression of FMNL2, Vimentin and E-Cadherin proteins in each group 72 h after transfection.Results The relative expression of FMNL2 mRNA and protein in both Hela cells and Siha cells of the experimental group 72 h after transfection was lower than that in the control group, respectively (P<0.05).The relative expression of Vimentin mRNA and protein in both Hela cells and Siha cells of the experimental group 72 h after transfection was lower than that in the control group, respectively, whereas the expression of E-cadherin mRNA and protein was higher than that in the control group (all P<0.05).Conclusions FMNL2 gene small interference RNA transfection may inhibit the expression of Vimentin and promote the expression of E-cadherin in Hela cells and Siha cells.FMNL2 may participate in the epithelial-mesenchymal transition of human cervical cancer.
Keywords：cervical neoplasmscervical carcinomacervical squamous-cell carcinomauterine cervical adenocarcinomaformin-like proteinepithelial-mesenchymal transition
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 5-8 )