Effects of CNP on renal function and renal apoptosis in rats with renal ischemia-reperfusion injury
PIAO Minhu
JIN Xiunan
LI Xiangdan
CUI Fengde
XU Dongyuan
Abstract：Objective To observe the effects of C-type natriuretic peptide (CNP) on renal function and renal tissue apoptosis in rats with renal ischemia-reperfusion (IR) injury and its mechanisms involved.Methods Twenty-four male Wistrar rats were randomly divided into the Sham group, IR group and CNP group (n=8).All the rats were left unilaterally nephrectomized.In the IR group and CNP group, we nipped the right renal artery and vein with noninvasive small vascular clip for 45 min.CNP [0.2 μg/(kg·min)] and 2 mL physiological saline was infused immediately and slowly at the start of a 45-min renal ischemia for 2 h in the two groups, respectively.The right renal artery and vein in the Sham group was not treated by the clip.Rats were killed to obtain the blood at 24 h after I/R.The serum creatinine (Cr) and urea nitrogen (BUN) was measured, cyclic guanosine monophosphate (cGMP) in right renal tissues was measured by ELISA, and the apoptotic cells in kidney tissues was detected by TUNEL staining.The renal tissue protein kinase B (Akt), β glycogen synthase kinase 3β (GSK-3β) protein and their phosphorylated protein was measured by Western blotting.Results Compared with the Sham group, the levels of serum Cr and BUN were significantly increased (both P<0.01), the concentration of cGMP in renal tissues was decreased, and the apoptosis rate was increased (both P<0.05), the protein phosphorylation levels of Akt and GSK-3β in the renal tissues were decreased in the IR group (both P<0.05).Compared with the IR group, the levels of serum Cr and BUN were decreased (both P<0.05), the concentration of cGMP was increased in the renal tissues and the apoptosis rate was decreased (both P<0.05), the protein phosphorylation levels of Akt and GSK-3β in renal tissues were increased in the CNP group (both P<0.01).Conclusions CNP can alleviate IR-induced kidney injury and renal tissue apoptosis.The mechanism may be related to the increased renal tissue cGMP levels which effectively increase Akt and GSK-3β protein phosphorylation.
Keywords：acute kidney injuryischemia-reperfusionC-type natriuretic peptiderenal functionapoptosisprotein kinase Bglycogen synthase kinase-3βrats
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 23-25 )