Variation of intestinal flora in patients with type 2 diabetes mellitus and the significance
MA Suxian
ZHANG Ruirui
WANG Su
DENG Jialiang
YANG Lu
YUAN Guoyue
Abstract：Objective To detect the variation of intestinal flora in patients with type 2 diabetes mellitus (T2DM) and the clinical significance.Methods The fecal samples were collected from 8 T2DM patients and 10 healthy participants.Total DNA was extracted from stool samples and submitted to high-throughput sequencing with primers targeting V4 region of the 16s rRNA gene.High-throughput sequencing was used to analyze the abundance and diversity of fecal microbiota.The difference of intestinal flora in patients with different gender, and the relationship between the dominant bacteria in the T2DM patients and fasting plasma glucose (FPG), 1 hour postprandial blood glucose (1h PG) and 2 hour postprandial blood glucose (2h PG) were investigated.Results The Shannon diversity index of T2DM patients and healthy subjects was 3.70±0.29 and 3.30±0.20, respectively, and there was no significant difference between the two groups (P>0.05).The dominant bacteria in the two groups were Firmicutes, Bacteroides, Proteobacteria, Actinobacteria at phylum level, and there was no significant difference in the abundance between the two groups (all P>0.05).In addition, the dominant bacteria were Bacteroides and Lachnospiraceae in the two groups at genus level.Compared with healthy subjects, the abundance of Roseburia was significantly reduced in the T2DM patients (P<0.05).The abundance of the Bacteroides in the female T2DM patients was much higher than that of the male patients, while the abundance of Peptostreptococcaceae was much lower than that of the male patients (P<0.05).The abundance of Faecalibacterium was positively correlated with 2 h PG (r=0.75, P<0.05), and the other dominant bacteria were not correlated with FPG, 1 h PG, 2 h PG (all P>0.05).Conclusion T2DM patients show intestinal flora imbalance with gender differences, and the abundance of Faecalibacterium in T2DM patients is related to blood glucose, which may provide a new therapeutic approach for T2DM.
Keywords：type 2 diabetes mellitusintestinal floraintestinal dominant fungiabundanceblood glucosehigh-throughput sequencing
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 20-23 )