Observation of proliferation and migration in nasopharyngeal carcinoma cells with over-expression of CYFIP1
LIN Zhongyuan
LI Yinyin
SHI Xiang
LI Wenchao
MO Lijun
LI Xiaohong
MO Wuning
Abstract：Objective To investigate the changes in the proliferation and migration of nasopharyngeal carcinoma cells with over-expression of cytoplasmic FMRP interacting protein 1 (CYFIP1). Methods A human nasopharyngeal carcinoma cell line (CNE2) was infected with a lentiviral vector containing CYFIP1 or the empty vector, which were used as the experimental group and blank group. The uninfected CNE2 cells acted as the control group. Cell proliferation was evaluated by MTT (represented by OD value) after cell culture for 24, 48, 72 and 96 h, separately. In clone formation assays, the cell culture was terminated when clones in plate could be seen by naked eyes and clones were counted. Cell migration was analyzed by Transwell chamber and Scratch assays. The cell migration distance and the number of penetrating cells were calculated. Results No significant difference was found in the OD value at different cell-culture time among these three groups (P>0.05). The number of clones in the experimental group, blank group and control group were 414±29, 426±30 and 381±59, respectively (P>0.05). The cell migration distance of the experimental group, blank group and control group was (6.71±0.1), (9.82±0.12) and (9.98±0.1) mm, respectively. Compared with the blank group and control group, the experimental group had a shorter migration distance (all P<0.05). The penetrating cells of the experimental group, blank group and control group were 37.33±1.53, 62.67±5.03 and 62.13±4.36. The experimental group had less penetrating cells than those of the blank group and control group (all P<0.05). Conclusions The cell migration of nasopharyngeal carcinoma cell line CNE2 with over-expression of CYFIP1 is inhibited. Abnormal expression of CYFIP1 maybe participates in the invasion and development of nasopharyngeal carcinoma.
Keywords：nasopharyngeal carcinomanasopharyngeal carcinoma cellscytoplasmic FMRP interacting protein 1cell proliferationcell migration
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 17-20 )