Effect of 1,25(OH)2D3 on apoptosis of human mesangial cells and its mechanism
GU Jie
ZHANG Hao
ZHANG Chunjiang
ZHAO Dan
LIU Gang
YANG Xiaoping
Abstract：Objective To investigate the effects of 1,25-dihydroxyvitamin D3[1,25(OH)2D3] on apoptosis and expression of apoptosis-related factors in human mesangial cells (HMC).Methods The cultured HMC were randomly divided into four groups: normal control group (group N,cultured in DMEM medium with 5% fetal bovine serum),epidermal growth factor (EGF) group (group E,cultured in DMEM medium plus 10 ng/mL EGF),1,25(OH)2D3 group (group V,cultured in DMEM medium plus 10-8 mol/L 1,25(OH)2D3),and the EGF+1,25(OH)2D3 group (group VE,cultured in DMEM medium plus 10 ng/mL EGF and 10-8 mol/L 1,25(OH)2D3),they were all treated for 48 h.The Caspase-3 activity was detected by the Casapase-3 kits.The protein expression of Akt,phosphorylated Aakt (p-Akt) Caspase-3,Caspase-8,and Caspase-9 was detected by Western blotting.Meanwhile,the mRNA expression of Caspase-3,Caspase-8,and Caspase-9 mRNA was detected by RT-PCR.Results The activity of Caspase-3 in the group E was lower than that in the group N,the activity of Caspase-3 in the groups V and EV was higher than that in the group N,and the activity of Caspase-3 in the group EV was higher than that in the group E (all P<0.05).The ratio of p-Akt/Akt in the group E was higher than that in the group N,and the ratio of p-Akt/Akt in the group V was lower than that in the group N,and the ratio of p-Akt/Akt in the group EV was lower than that in the group E (all P<0.05).The protein and mRNA expression of Caspase-3 and Caspase-9 in the group E was lower than that in the group N,the expression of Caspase-3 and Caspase-9 in the group V was higher than that in the group N,and the expression of Caspase-3 and Caspase-9 in the group EV was higher than that in the group E (all P<0.05).Conclusion 1,25(OH)2D3 may enhance the apoptosis activity of HMC and thus promote the apoptosis of HMC by up-regulating the expression of Caspase-3/9 and down-regulating the expression of p-Akt.
Keywords：human mesangial cells125-Hydroxyvitamin D3epidermal growth factorapoptosisprotein kinase Bcysteine protease
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 1-5 )