Effects of miR-24 on cell proliferation, metastasis and autophagy of HUVECs

doi:10.3969/j.issn.1002-266X.2017.13.007

Abstract：To investigate the effects of miR-24 on the cell proliferation, metastasis and autophagy of human umbilical vein endothelial cells (HUVECs).Methods HUVECs were randomly divided into the blank control group, rapamycin + miR-24 high expression group and rapamycin group.The control group was not treated.HUVECs in the rapamycin group were treated with 1000 nmol/L rapamycin for 6 h to establish an autophagic model.HUVECs in the rapamycin + miR-24 high expression group were transfected with miR-24 high expression plasmid.After successful transfection, the cells were treated with 1 000 nmol/L rapamycin for 6 h to establish the autophagic models.The proliferation ability of HUVECs was detected by CCK-8, and the cell migration ability was measured by cell scratch test, the protein expression levels of microtubule-associated protein 1 light chain 3 (LC3) and Beclin-1 in HUVECs were detected by immunohistochemistry and Western blotting.The autophagic bodies were observed by transmission electron microscopy.Results Compared with the blank control group, the OD value and migration ratio of the rapamycin group and the rapamycin + miR-24 high expression group were decreased and the expression of Beclin-1 protein and LC3 II protein was increased (P<0.01 or P<0.05).Compared with the rapamycin group, the OD value and migration ratio of the rapamycin + miR-24 high expression group were decreased, and the expression of Beclin-1 and LC3 II protein was decreased (P<0.01 or P<0.05).The results of transmission electron microscopy showed that the distribution of each organelle in the cytoplasm of the blank control group and the rapamycin + miR-24 high expression group was normal, the nucleus morphology was normal and the autophagic bodies were not found in the cytoplasm.In the the rapamycin group, the obvious vacuolar structure and the autophagosome containing part of the bilayer structure conld be seen in the cytoplasm.Conclusion miR-24 significantly inhibits the proliferation, metastasis and autophagy of HUVECs.