Effect of activating transcription factor 2 on cell proliferation and cell cycle of lung carcinoma AG490 cells
GAO Shan
LI Zhenhua
GENG Shuang
ZHAO Su
Abstract：Objective To investigate the effect of activating transcription factor 2 (ATF2) on cell proliferation and cell cycle of lung carcinoma AG490 cells.Methods Using PCR to synthesize the ATF2, employing third generation lentivirus packaging system to get the control virus and ATF2 overexpression virus.The lung cancer cell line AG490 was randomly divided into the control group and the observation group, which was infected by the control virus and ATF2 overexpression virus.Using qRT-PCR to detect the expression of ATF2 in mRNA level, and the cell cycle was detected by flow cytometry.The cell proliferation index was detected by CCK-8 kit at 1, 2, 3 and 4 days after infection.The relative expression of ATF2, p53 and p21 protein was detected by Western blotting at 48 h.Results After infecting the lung carcinoma cell for 48 h by using control and ATF2 virus, the expression level of ATF2 mRNA and ATF2 protein increased in the observation group as compared with that of the control group (all P<0.01).The proliferation index on the day 4 of the observation group was higher than that in the control group (P<0.05).The expression of P53 and P21 protein in observation group was higher than that in the control group (both P<0.01).Conclusion ATF2 can promote the cell proliferation, activation of the cell cycle in lung carcinoma cell line AG490 by inhibiting P53 and P21 protein expression.
Keywords：lung carcinomaactivating transcription factor 2cell proliferationcell cycle
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 27-29 )