Levels of lymphoma-associated macrophages in bone marrow of patients with diffuse large B-cell lymphoma and its clinical significance
WANG Yan
XING Limin
XU Wenyan
WANG Huaquan
SU Xin
SHAO Zonghong
Abstract：Objective To investigate the levels of lymphoma-associated macrophages (LAM) in bone marrow of patients with diffuse large B-cell lymphoma (DLBCL) and its clinical significance.Methods Forty DLBCL patients (DLBCL group) and 18 healthy controls (NC group) were enrolled in the study.M2 macrophages (LAM) in the bone marrow and the ratio of M2 to M1 (M2/M1) in the two groups were detected by flow cytometry (FCM).C-C motif chemokine 22 (CCL22) mRNA expression was analyzed using RT-PCR.The relationship between the percentage of M2 macrophages in bone marrow and CCL22mRNA, lactate dehydrogenase (LDH), β2 microglobulin (β2-MG) was analyzed by Pearson correlation analysis.Results The M2 percentages in the DLBCL group and NC group were 11.38%±1.23% and 4.53%±0.83% , respectively (P<0.05).The M2/M1 ratios in the DLBCL group and NC group were 0.75±0.08 and 0.61±0.07, respectively (P<0.05).The CCL22 mRNA expression in the bone marrow mononuclear cells of the DLBCL group and NC group was 4.64±0.73 and 2.12±0.76, respectively (both P<0.05).The M2 percentage and M2/M1 ratio and the CCL22 mRNA expression in the bone marrow mononuclear cells of patients with initial treatment and progression of disease (PD) were higher than those in the effectively treated and stable disease (SD) patients (both P<0.05).The M2 percentage in the IPI score 0-1 group was lower than in the IPI score ≥2 group (P<0.05).The M2 percentage in stage I-III patients was lower than that in the stage Ⅳ patients (P<0.05).The M2 percentage in patients with B symptom was higher than in patients without B symptom (P<0.05).There were positive correlations between the M2 percentage and the CCL22 mRNA expression, LDH level and β2-MG level (r=0.37, 0.49, 0.42, all P<0.05).Conclusion The increased amount of LAM in bone marrow of patients with DLBCL is related to disease progression, curative effect and prognosis, which can reflect the tumor burden and promote the expression of CCL22.
Keywords：diffuse large-B-cell lymphomalymphoma-associated macrophagesC-C motif chemokine 22
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 16-19 )