Association between PRSS1 gene polymorphism and chronic pancreatitis in southwestern Chinese Han population

ZHU Yutian; YAO Wuxiu; WANG Yang; YAN Maolin; CHEN Heping

doi:10.3969/j.issn.1002-266X.2017.10.006

Abstract：Objective To investigate the association between PRSS1 single nucleotide polymorphism (SNP) and chronic pancreatitis (CP) in southwestern Chinese Han population.Methods Genomic DNA was prepared from 384 individuals with CP (CP group) and 480 healthy controls (control group).Three SNPs in the PRSS1 gene (rs10273639,rs6667 and rs2011216) were selected by case-control study.The SNPs were detected by single-base extension method and their correlation with CP was analyzed.Results All three SNPs conformed to Hardy-Weinberg equilibrium (all P ＞ 0.05).The G allele frequencies of rs6667 and rs2011216 were 37.8％,33.2％,29.8％ and 26.5％ in the CP group and control group,respectively.The GG genotype frequencies were 14.1％,10.4％,7.5％ and 6.0％ (all P ＜ 0.05).The allele and genotype of rs10273639 had no significant difference between the CP group and control group (all P ＞ 0.05).Binary logistic regression analysis showed that the risk of CP in rs6667 G allele was 1.18 times more than that in A allele,while in the dominant model [(AG + GG) vs AA] and recessive model [GG vs (AG + AA)] it was 1.18-fold and 1.76-fold higher in CP group than that of the control group,respectively.In the risk of developing CP,rs2011216 G allele carriers were1.16 times as high as the A allele carriers.In the dominant model [(AG + GG) vs AA] and recessive model [GG vs (AG + AA)],the risk of CP group was 1.17 times and 1.34 times higher than that of the control group,respectively.In addition,rs10273639 was not associated with CP susceptibility (P ＞ 0.05).Conclusion The rs6667 and rs2011216 loci of the PRSS1 gene may be associated with chronic pancreatitis among southwestern Chinese Han population.