Effects of Wnt signaling pathway antagonist DKK-1 on invasion ability of colon cancer cell line HCT116
ZHANG Yanxin
ZHANG Yinpo
ZHAO Yan
WANG Jihong
ZHANG Yan
CUI Dongtao
Abstract：Objective To investigate the effects and molecular mechanism of Wnt signal pathway antagonist DKK-1 on invasion ability of colon cancer cell line HCT116.Methods Conlon cancer HCT116 cells were divided into the experimental group and control group,which were cultured with exogenous DKK-1 (100 ng/mL) and the same amount of cell culture medium for 48 h,respectively.Transwell was used to observe the change of invasion ability.The protein and mRNA expression of β-catenin,Snail,E-cadherin and Vinentin in the HCT116 cells was observed by Western blotting and RT-PCR.Results The number of transmembrane cells in the experimental group was (45.29 ± 6.25)/HP which was obviously lower than that in control group [(76.36±8.32)/HP],and the difference was statistically significant (P ＜ 0.05).The protein and mRNA expression of β-catenin,Snail and Vinentin was lower and the expression of E-cadherin protein and mRNA was higher in the experimental group than that of the control group,and the differences were statistically significant (all P＜0.05).Conclusion Wnt signal pathway antagonist DKK-1 can decrease the invasion ability of conlon cancer cell line HCT116 by inhibiting the process of epithelial-mesenchymal transition in tumor cells.
Keywords：colon carcinomaWnt signaling pathwaypathway antagonistepithelial-mesenchymal transitioncell invasion
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 8-10 )