Reversing effects of total glucosides of paeony on drug resistance of BEL-7402/ADM cells

HU Liyi; HOU Yongbin; YU Lihua; MI Yonghua; WANG Ke; LIU Beizhong

doi:10.3969/j.issn.1002-266X.2017.10.001

Abstract：Objective To observe the reversing effects of total glucosides of paeonia (TPG) on drug resistance of BEL-7402/ADM cells in hepatocellular carcinoma.Methods Human hepatocellular carcinoma ADM sensitive BEL-7402 cells (parent group) and ADM drug-resistant BEL-7402/ADM cells (drug resistance group) were cultured in vitro.BEL-7402/ADM cells were transfected with HDAC3 siRNA interference plasmid (interference group) and empty plasmid (empty plasmid group) for 24 h,respectively,and BEL-7402/ADM cells were incubated with TPG for 48 h (drug group).Different concentrations of ADM were administered to cells in the parent group,drug resistance group and drug group for 48 h respectively.Then MTT assay was used to detect the growth inhibition rates and IC50 of ADM in each group.The mRNA and protein expression of MDR1 and histone deacetylase 3 (HDAC3) was detected by RT-qPCR and Western blotting in the above grovps.Results IC50 of ADM was 0.139 nmoL/L in the parent group,8.807 nmoL/L in the drug resistance group and 4.890 nmoL/L in the drug group.Drug reverse ratio was about 1.80 times higher and the reverse transcription ratio was 45.23％ in the drug group.The relative expression of MDR1 mRNA and protein was:drug resistance group,empty plasmid group ＞ drug group ＞ interference group ＞ parent group (all P＜0.05,0.01),the relative expression of HDAC3 and protein was:drug resistance group,empty plasmid group ＞ interference group ＞ parent group,drug group (all P＜0.05,0.01).Conclusion TPG induces the down-regulated expression of MDR1 by inhibiting the HDAC3 expression,thus reversing the drug resistance of BEL-7402/ADM cells.