Expression changes of CD62L, BAFF and CD40L in basophils of patients with systemic lupus erythematosus

FENG Yongmin; CHEN Qiuping; HUANG Liutao; PAN Qingjun; LIU Huafeng

doi:10.3969/j.issn.1002-266X.2017.09.006

Abstract：Objective To investigate expression changes of lymph homing receptor (CD62L and B-lymphocyte activation factors (BAFF and CD40L) in peripheral blood basophils of patients with active systemic lupus erythematosus (SLE) and their significance.Methods Fifty-seven SLE patients were selected and were divided into the active group (SLEDAI score≥10,n =29) and inactive group (SLEDAI score ＜ 10,n =28) according to the SLEDAI score.Twenty healthy volunteers were selected as the control group.The levels of CD62L,BAFF and CD40L in the peripheral blood basophils were detected by flow cytometry and their correlations with the Serum anti-dsDNA levels were analyzed by Spearman correlation analysis.Results The expression of CD62L in peripheral blood basophils of the active group and inactive group was higher than that of the control group,and the active group was significantly higher than the inactive group (P ＜0.05).The expression of BAFF and CD40L in peripheral blood basophils of the active group was significantly higher than that of the inactive group and control group (all P ＜ 0.05),while there was no significant difference between inactive group and control group (P ＞ 0.05).Expression of CD62L and CD40L in the peripheral blood basophils was positively correlated with serum anti-dsDNA levels in SLE patients (r =0.34,r =0.36;all P ＜ 0.05).The expression of BAFF in peripheral blood basophils was not related with serum anti-dsDNA levels in SLE patients (r =0.26,P =0.14).Conclusions Expression of CD62L,BAFF and CD40L in basophils of patients with active SLE increases.CD62L may mediate the basophils migrating to the secondary lymph organs where the basophils could activate the self-reactive B-lymphocytes producing diverse antibodies through BAFF and CD40L to participate in the pathogenesis of SLE.