Diagnostic value of serum HBcrAg and linearized HBsAg for occult hepatitis B virus infection
ZHU Changlin
XUE Xiongyan
CHEN Zhanze
GU Yuyu
XU Yangyang
LI Weixuan
Abstract：Objective To investigate the value of serum hepatitis B virus core-related antigens (HBcrAg) and linearized hepatitis B surface antigen (HBsAg) in diagnosis of occult hepatitis B virus infection (OBI) in Chinese population.Methods HBcrAg and linearized HBsAg levels were measured by chemiluminescence enzyme immunoassay (CLEIA) in 63 patients with OBI (OBI group) and 150 healthy people (control group).The serum HBV DNA was detected by fluorescence quantitative PCR.Receiver operating characteristics (ROC) was analyzed to calculate diagnostic accuracy of HBcrAg and combination with linearized HBsAg.Results Serum HBcrAg and linearized HBsAg were detected in 90.5％ and 42.9％ of the OBI group,and 10.7％,6.0％ of the control group,respectively (x2 =125.5,42.9,all P ＜ 0.05).There were positive correlation between HBV DNA and HBcrAg,HBV DNA and linearized HBsAg,HBcrAg and linearized HBsAg,respectively (R2 =0.471 4,0.122 2,0.086 8,P ＜ 0.05 or P ＜ 0.01).HBcrAg had better area under the ROC curves (AUC) [0.911 (95％ CI:0.864-0.946)] than linearized HBsAg [0.683 (95％ CI:0.616-0.745),Z=6.134,P ＜ 0.05].The AUC of combined detection was 0.895 (95％ CI:0.846-0.933),and no significant improvement was found (Z =0.657,P ＞ 0.05).The optimal HBcrAg and linearized HBsAg cutoff values were 100 U/mL and 0.48 IU/mL,respectively.The sensitivity of HBcrAg in diagnosis of OBI was significantly higher than that of linearized HBsAg (x2 =121.4,P ＜ 0.01),while specificity was a little lower (x2 =3.034,P ＞ 0.05).Combined detection in diagnosis of OBI did not show any improvement than single detection of HBcrAg or linearized HBsAg in sensitivity or specificity (x2 =1.322,3.802,all P ＞ 0.05).Conclusion There is a good correlation between serum HBcrAg and HBV DNA,and HBcrAg is more sensitive than linearized HBsAg or combined detection in diagnosis of OBI,which can be used as a good serological marker for the diagnosis of OBI.
Keywords：occult hepatitis B virus infectionHepatitis B virus core-related antigenslinearized hepatitis B surface antigen
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 9-12 )