Effect of piplartine on radio-sensitization of human pulmonary adenocarcinoma A549 cells
XIAO Nan
XU Danghui
PAN Zhiyao
YAO Zhifeng
Abstract：Objective To investigate the effect of piplartine on radio-sensitization of human pulmonary adenocarcino-ma A549 cells.Methods A549 cells cultured in vitro were treated with 1, 2, 4, 8 and 16 μmol/L piplartine for 24, 48 and 72 h.CCK8 assay was used to measure the proliferation inhibition of piplartine on A 549 cells in each group and then we selected the minimum concentration which had no obvious toxic effect on A 549 cells.A549 cells were divided into four groups:the control, piplartine group, radiation group and the combination treatment group .The control group was not trea-ted.The piplartine group was given the concentration of 1μmol/L piplartine , the radiation group was given X-ray radiation of 8 Gy alone, and the combination treatment group was given the concentration of 1 μmol/L piplartine for 48 h, then was exposed to X-ray radiation of 8 Gy.CCK8 assay was used to measure the proliferation inhibition of piplartine on A 549 cells in each group.Cell cycle and apoptosis of A549 cells were analyzed by flow cytometry (FCM).Results After being trea-ted with piplartine for 24, 48 and 72 h, the proliferation inhibition rate of the piplartine group was lower than that of the other groups (all P<0.05).The proliferation inhibition in the combination treatment group was higher than that of the ra -diation group and the piplartine group at 48 h (all P<0.05).The percentage of cells in the G0/G1 phase of the combina-tion treatment group was significantly increased , and was decreased in the S phase , and apoptosis rate was also higher than that of the radiation group (P<0.05).Conclusion Piplartine at the concentration of 1 μmol/L can enhance the radio-sensitivity of A549 cells, which may be associated with the enhancement of radiation-induced inhibition of cell proliferation and induction of apoptosis as well as cell arrest in G 0/G1 phase.
Keywords：piplartinepulmonary adenocarcinomaX-rayradio-sensitizationcell proliferationcell cycleapopto-sis
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 13-16 )