Effect of curcumin on proliferation and CDH13 methylation status of non-small-cell lung cancer cell line A549

ZHAO Ying; LI Yingzi

doi:10.3969/j.issn.1002-266X.2017.06.003

Abstract：Objective To observe the effect of curcumin on DNA methyltransferase (Dnmts) activity and tumor sup-pressor gene human H-cadherin (CDH13) in non-small-cell lung cancer (NSCLC) A549 cells and to explore the mecha-nism of curcumin's demethylation .Methods A549 cells were divided into two groups:the observation group and the con-trol group.Cells in the observation group were cultured with 40 μmol/L curcumin and the culture medium , while cells in the control group only with the culture medium .MTT assay was used to detect the cell proliferation , and RT-PCR was used to detect the expression of CDH 13 mRNA.CpG island methylation status of CDH 13 promoter was detected by methylation-specific PCR ( MSP) , and catalytic activity of Dnmts was detected by enzyme colorimetric determination kit .Results The OD of the observation group was 0.638 ±0.184, which was lower than that (0.859 ±0.040) of the control group (P<0.05).The relative expression level of CDH13 mRNA in the observation group was 0.64 ±0.09, which was higher than that (0.43 ±0.12) of the control group.There were methylation amplification bands , but no demethylation bands in the control group, and there were both methylation amplification bands and demethylation bands in the observation group .In the observation group and the control group , the activities of Dnmt1 were separately (0.153 ±0.016) and (0.770 ± 0.014) μmol/min, and the activities of Dnmt3b were (0.137 ±0.020) and (0.179 ±0.013) μmol/min, respectively. The activities of Dnmt1 and Dnmt3b in the observation group were lower than those in the control group (all P<0.05). Conclusion Curcumin inhibits the methylation status of CDH 13 by decreasing the activity of Dnmts , and thereby inhibits A549 cell proliferation .