Regulatory effect of glucagon on insulin secretion of pancreatic βcell-MIN6 cells
SHI Yanqiu
LI Jun
LI Siyuan
ZHANG Zhen
Abstract：Objective To explore the regulation effect and mechanism of glucagon on insulin secretion of the pancre-atic βcell-MIN6 cells.Methods The cultured MIN6 cells were transfected with ICUE3,a fluorescent biosensor DNA sensitive to cyclic adenosine monophosphate (cAMP),and then were randomly divided into three groups,respectively, which were cultivated for 400-600 s under the glucose-free,low-glucose (2.8 mmol/L glucose)and high-glucose (16.7 mmol/L glucose)conditions.The cells in each group were then randomly divided into four groups:0,100,500,1000 ng/L groups which were treated with 0,100,500,1000 ng/L glucagon for 350-600 s.Using fluorescence resonance ener-gy transfer technology (FRET)to detect the cAMP levels in MIN6 cells,and the Ins secretion was measured by enzyme-linked immune sorbent assay (ELISA).Results Under the glucose-free condition,the levels of cAMP and Ins secretion in MIN6 cells of the 1000 ng/L group were significantly higher than those in the 500 ng/L group,and significant difference was found between every two groups (all P <0.05 ).Under the low-glucose and high-glucose conditions,the levels of cAMP and Ins secretion in MIN6 cells:1000 ng/L group >500 ng/L group >100 ng/L group >0 ng/L group,signifi-cant difference was found between every two groups (all P <0.05).And under the high glucose environment,the differ-ence was more significant.Conclusion Glucagon may stimulate insulin secretion by increasing cAMP levels in the way of concentration gradient within the islet βcell line-MIN6 cells,and this trend is glucose-dependent.
Keywords：glucagoninsulinpancreatic βcellsMIN6 cellscyclic adenosine monophosphate
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 19-21 )