Inhibitory effect of miR-497-5p on proliferation of human cervical carcinoma cell
ZHANG Jun
GAO Lanyang
ZHAN Ping
MAO Xiguang
Abstract：Objective To investigate the inhibitory effect and mechanism of miR-497-5p on proliferation of human cervical cancer HeLa cells.Methods The human cervical cancer HeLa cells in the logarithmic growth phase were random-ly divided into two parts.The first part was randomly divided into 4 groups:miR-497-5p mimics NC and IKCa1-wt co-transfection group,miR-497-5p mimics and IKCa1-wt co-transfection group,miR-497-5p mimics NC and IKCa1-mut co-transfection group and miR-497-5p mimics and IKCa1-mut co-transfection group which were transfected by corresponding plasmids for 48 h.The luciferase activity of HeLa cells was detected by double luciferase reporter assay system.The second part was randomly divided into 3 groups:miR-497-5p mimics transfection group,miR-497-5p mimics NC transfection group and blank control group.Cells in each group were transfected with Lipofectamin 2000.The expression of IKCa1 mRNA was detected by qRT-PCR,IKCa1 protein expression was detected by Western blotting and the proliferation ability of HeLa cells was measured by CCK-8 assay.Results The luciferase activity of miR-497-5p mimics and IKCa1-wt co-transfection group was lower than that of the other three groups of the first part (all P <0.05),and no significant difference among other three groups (all P >0.05),which confirmed that miR-497-5p and IKCa13 '-URT had specific binding sites.The expression of IKCa1 mRNA and protein of the miR-497-5p mimics transfection group was lower than that of the other two groups of the second part (all P <0.05),and there was no significant difference between the other two groups (all P >0.05).The pro-liferation ability of the miR-497-5p mimics transfection group was lower than that of the other two groups at 24,48,72 and 96 h of culture (all P <0.05),and there was no significant difference between the other two groups (all P >0.05).Con-clusion miR-497-5p can inhibit the proliferation of cervical cancer HeLa cells by negatively regulating the expression of IKCa1 gene.
Keywords：cevical carcinomamicro RNA-497-5pintermediate-conductance-Ca2 +-activated K +channelIKCa1 geneHeLa cellscell proliferation
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 15-18 )