Changes of chemosensitivity of crizotinib-resistant cells H2228/CR to paclitaxel and cisplatin
GAO Haixiang
Abstract：Objective To observe the sensitivity changes of lung adenocarcinoma H 2228 cells to paclitaxel and cispla-tin before and after resistance to crizotinib , and to explore the molecular mechanism .Methods Crizotinib-resistant H2228/CR cells were induced by continuous stepwise exposure to crizotinib combined with pulse therapy in vitro .MTT as-say was used to measure the growth inhibition ratio of H 2228 and H2228/CR cells treated with different concentrations of crizotinib, paclitaxel and cis-platinum.Real-time fluorescence quantification PCR and Western blotting were used to meas-ure the mRNA and protein expression of Vimentin , N-cadherin and E-cadherin in H2228 and H2228/CR cells. Results After being cultured for 6 months, H2228/CR cell displayed significantly resistance to crizotinib and its resist-ance to paclitaxel and cisplatin was decreased .The mRNA and protein expression of E-cadherin was decreased in H 2228/CR cells as compared with that of the parental cell line H 2228, while the mRNA and protein expression of N-cadherin and Vimentin was increased in H2228/CR cells.Conclusion The crizotinib-resistant H2228/CR cells decrease sensitivity to standard chemotherapeutic drugs paclitaxel and cisplatin , which may be associated with the epithelial-mesenchymal transi-tion of H2228/CR cells.
Keywords：non-small-cell lung cancerdrug resistancecrizotinibpaclitaxelcisplatin
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 25-28 )