Construction of human Tudor-SN UTR luciferase reporter gene expression plasmid and its activity detection
ZHAO Yali
SU Chao
GAN Shihu
REN Yuanyuan
GAO Xingjie
YANG Jie
HE Jinyan
Abstract：Objective To lay a foundation for further study on the mechanism of Tudor-SN protein in translation . Methods The human Tudor-SN UTR fragments were amplified by PCR from genomic DNA extracted from HeLa cells , and then were inserted into pGL 3-Control expression vector .HeLa cells were co-transfected with the renilla luciferase plasmid and recombinant pGL3-5′UTR plasmid or pGL3-3′UTR plasmid.After 48 hours, luciferase activity was detected . Results Both double enzyme digestion and gene sequencing confirmed the construction of recombinant plasmid was suc -cessful.The luciferase activity was detected after transfection , and the luciferase activity of pGL3-5′UTR plasmid was the best.Conclusion The recombinant plasmids of pGL 3-5′UTR-luciferase and pGL3-3′UTR-luciferase are successfully con-structed, which lays a foundation for further study of regulatory mechanisms of Tudor -SN genetic UTR in translation .
Keywords：human Tudor-SN protein5′UTR3′UTRrecombinant plasmidluciferase
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1-4 )