Prediction of miR-210 erythroid cells-related downstream target genes

SHI Jiyu; LIU Fang; DING Jin; CAO Yan; LI Wenjun

doi:10.3969/j.issn.1002-266X.2017.03.006

Abstract：Objective To predict the downstream target genes related to miR-210 erythroid cells by bioinformatics. Methods NCBI was used to analyze the conservation of the mature sequence of miR-210 in mammals.The online database of bioinformatics was applied to predict miR-210 downstream target genes.The predicted miR-210 downstream target genes were analyzed by GO classification and enrichment using the BINGO insert in Cytoscape.Screening miR-210 downstream tar-get genes involved in erythroid proliferation,differentiation and apoptosis.The downstream target genes of miR-210 were pre-dicted by DAVID and KEGG database after signal transduction pathway enrichment analysis.Targetscan online tools were used to verify the interaction of target genes with miR-210.Results There was a highly conserved nature of miR-210 mature sequence in many species.The number of the predicted target genes highly related to miR-210 was 416.The molecular func-tions of target genes were enriched in protein binding,transmembrane transport activity of ion,enzyme binding and other mo-lecular functions.The biological process were mainly cell signal transduction,synaptic transmission,etc.,and the composi-tions of the cell components were synaptic vesicles,cytoplasm,organelles and so on.We screened 26 miR-210 downstream target genes which were closely related to erythroid proliferation,differentiation and apoptosis.The signal pathways involved in the regulation were mainly cancer signaling pathway,PI3K-Akt signaling pathway and so on.Smad3,Mapk10,Stat5a, Ywhag,Ctgf and Kitl6 genes were closely related to erythroid proliferation and differentiation,during which,Smad3, Mapk10,Stat5a,Ywhag and Kitl might be directly regulated by mir-210,and Ctgf might be indirectly regulated by miR-210. Conclusions Smad3,Mapk10,Stat5a,Ywhag,Ctgf and Kitl6 are initially screened for the downstream target genes related to miR-210 erythroid cells.These target genes may be related to the erythroid proliferation and differentiation.