Therapeutic effect of Wharton jelly-derived mesenchymal stem cell transplantation on recurrent spontaneous abortion

WU Rongrong; ZHANG Yuquan; XU Yunzhao; CHEN Xiaojing; GAO Xiaoyue; YANG Xiaoqing

doi:10.3969/j.issn.1002-266X.2017.03.003

Abstract：Objective To investigate the therapeutic effect of Wharton jelly-derived mesenchymal stem cells (WJ-MSCs)on recurrent spontaneous abortion (RSA)and its mechanism.Methods Female CBA /J mice were separately ma-ted with male BALB/c and DBA /2 mice to construct normal pregnant (n =5)and RSA models (n =20).The RSA models were randomly divided into the model group,experimental groups 1,2 and 3,five mice in each group.Mice in the model were injected with 0.2 mL DMEM/F12 culture medium,mice in the experimental groups were injected with WJ-MSCs (6 ×107 /mL)by tail vein on day 1 of pregnancy,The mice in the experimental group 1 were injected once,mice in the ex-perimental group 2 were injected every other day for 2 times,and mice in the experimental group 3 were injected every other day for 3 times.The mice were killed on day 14 of pregnancy,and we calculated the abortion rate,and their decidual and placental tissues were obtained to detect the expression of IL-10,IL-17 and IFN-γby Western blotting and qRT-PCR,re-spectively.Results The embryo resorption rates in the model group and experimental group 1 were higher than that of the control group,and the embryo resorption rate in the experimental group 3 was lower than those in the model group and ex-perimental group 1 (all P <0.05).The protein expression of IL-17 in the experimental groups 1 and 2 was higher than that in the control group,while it was lower in the experimental group 3 than that of the control group (all P <0.05).The rela-tive mRNA expression of IFN-γin the experimental group 1 was higher than that in control group,while in the experimental groups 2 and 3,the levels were lower than that in the control group (all P <0.05).The relative mRNA and protein expres-sion of IL-10 in the experimental groups 1,2 and 3 was higher,and the relative mRNA and protein expression of IL-17 and IFN-γwas lower than that in the model group (all P <0.05).The mRNA and protein expression of IL-10 in the decidua and placenta was gradually increased,the expression of IL-17 mRNA,protein and IFN-γprotein was gradually decreased in experiment groups 1,2 and 3.No significant difference was found in the IFN-γmRNA expression between the experimental groups 2 and 3,but significant difference was found in the rest of the indicators (all P <0.05)..Conclusions The em-bryo resorption rate in RSA mice is decreased after transplantation of WJ-MSC,which may be related to its up-regulation of IL-10 and down-regulation of IFN-γand IL-17.