Effect of pigment epithelium-derived factor on expression of CD36 protein on surface of macrophages

LU Jianjun; ZHANG Yongtao; NI Mei; LYU Huixia

doi:10.3969/j.issn.1002-266X.2017.01.002

Abstract：Objective To determinate the effect of pigment epithelium-derived factor (PEDF)on the expression of CD36 protein on the surface of macrophages in mouse atherosclerotic plaque and RAW264.7 cells.Methods In vivo,20 male ApoE-/-8-week-old mice were randomly divided into two groups:the control group and PEDF group.Ten mice in PEDF group were intravenously injected with PEDF to achieve high-level PEDF expression,and ten mice in the control group were given same amount of saline solution.The mice were put to death after 16-week high-fat feeding.The aortic root tissues were removed and immunohistochemistry was applied to evaluate the expression of CD36 protein on the surface of macrophages.In vitro,RAW264.7 cells were cultured in Dulbecco's Modified Eagle Medium (DMEM)with high glucose. After 24 hours,the RAW264.7 cells were divided into four groups:ox-LDL group,ox-LDL +negative vector (G)group, ox-LDL +PEDF group,and Con group.In addition to the Con group,the other three groups were given 80 μg/mL ox-LDL.A adenovirus vector containing a mouse PEDF expression sequence was constructed and transduced into ox-LDL +PEDF group with MIC value of 30.CD36 expression in the four groups was assessed by Western blotting.Results The expression of CD36 protein on the surface of macrophages in aortic root plaque of the PEDF group was 14.00 ±1.155, which was lower as compared with that of the control group 29.00 ±0.577,P <0.05.In vitro,the expression of CD36 protein in the ox-LDL group,ox-LDL +G group,ox-LDL +PEDF group,and Con group was 0.754 ±0.055,0.831 ± 0.072,0.547 ±0.002,and 0.339 ±0.033,respectively.Compared with Con group,the expression of CD36 protein in the ox-LDL group and ox-LDL +G group was increased (all P <0.05).Compared with ox-LDL group and ox-LDL +G group,the expression of CD36 protein in the ox-LDL +PEDF group was decreased (all P <0.05).Conclusion PEDF can effectively down-regulate CD36 expression on the surface of macrophages,which suggests that PEDF may serve as a po-tential target in the treatment of atherosclerosis. <br> 0.072,0.547 ±0.002,and 0.339 ±0.033,respectively.Compared with Con group,the expression of CD36 protein in the ox-LDL group and ox-LDL +G group was increased (all P <0.05).Compared with ox-LDL group and ox-LDL +G group,the expression of CD36 protein in the ox-LDL +PEDF group was decreased (all P <0.05).Conclusion PEDF can effectively down-regulate CD36 expression on the surface of macrophages,which suggests that PEDF may serve as a po-tential target in the treatment of atherosclerosis.