Altered expression levels of miRNAs in joint fluid of knee osteoarthritis patients
MU Shangqiang
SUN Shuang
WANG Ning
MEI Jiwen
Abstract：Objective To investigate the differential expression of miRNAs in joint fluid of patients with knee osteoarthritis (KOA) and its clinical significance.Methods Thirty-three female KOA patients and 33 female health people were chosen.Three cases of each group were randomly selected and the joint fluid samples of these study subjects were collected.The miRNA microarray was used to assess the level and composition of miRNAs in joint fluid.The differences criteria in screening expression of miRNA:up-regulated or down-regulated expression of miRNAs ≥ 2 folds.Real-time PCR was used to assess the relative expression of miRNAs in joint fluid of the remaining KOA patients and healthy subjects (30 cases in group).Results Compared with normal controls,3 miRNAs in which the up-regulated expression was ≥ 2 folds (miR-9,miR-155,and miR-98) and4 miRNAs in which the down-regulated expression was ≥ 2 folds (miR-140,miR-27a,miR-146a,and miR-138) were found in the KOA group.The expression levels of miR-9,miR-155 and miR-98 were higher in KOA patients than those in healthy subjects,while the expression levels of miR-140,miR-27a,miR-146a and miR-138 were lower in KOA patients than those in healthy subjects (all P ＜0.05).Conclusion The expression of miR-9,miR-155 and miR-98 in joint fluid of KOA patients is up-regulated,and the expression of miR-140,miR-27a,miR-146a and miR-138 is down-regulated,and these differentially expressed miRNAs may be related to the pathogenesis of KOA.
Keywords：osteoarthritiskneejoint fluidmicro RNA microarray
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 5-7 )