Changes in proliferation,migration and invasion abilities of oral squamous-cell carcinoma SCC1 5 cells transfected by miR-21 4 inhibitor and its mechanism

XU Chiqiang; XIAO Jingang

doi:10.3969/j.issn.1002-266X.2016.47.003

Abstract：Objective To investigate the changes in proliferation,migration and invasion abilities of oral squamous-cell carcinoma SCC15 cells transfected by miR-214 inhibitor,and to explore the mechanism.Methods The oral squa-mous-cell carcinoma SCC15 cells cultured in vitro were randomly divided into the observation group,negative control group and blank control group.SCC15 cells in the observation group and negative control group were transfected with miR-214 in-hibitor and inhibitor-NC in vitro by using Lipofectamine2000,respectively,and SCC15 cells in the blank control group were not given any intervention.The real-time PCR was used to detect the expression of miR-214 at 24 h after transfection. CCK-8 assay was used to observe the proliferation of the cells at 24,48 and 72 h after transfection.Scratch test and Tran-swell assay were used to observe the migration and invasion abilities of the cells at 24 h after transfection.Western blotting was used to explore the expression of Dikkopf-related protein-3 DKK-3 andβ-catenin protein at 24 h after transfection.Re-sults At 24 h after transfection,compared with the negative control group and blank control group,the expression of miR-214 and β-catenin protein and wound healing rate was significantly lower,the number of penetrating cells was significantly less and the expression of DKK-3 protein was significantly higher in the observation group (all P<0.05).The A450 in the observation group was lower than that in the negative control group and blank control group at 48 and 72 h after transfection (all P<0.05 ).Conclusion The proliferation,migration and invasion abilities of oral squamous-cell carcinoma SCC15 cells transfected with miR-214 inhibitor decrease,and the mechanism may be that the transfection of miR-214 inhibitor can inhibit the expression of DKK-3 andβ-catenin protein in Wnt/β-catenin signaling pathway.