Effects of Prohibitin 1 gene silencing on spreading,migration and invasion of melanoma cells
BI Jiajia
Abstract：Objective To investigate the effects of prohibitin 1 (PHB)silencing on spreading,migration and inva-sion of melanoma A375 cells by RNA interference (RNAi).Methods The A375 cells were divided into the control group and experimental group.The cells in the control group were cultured in DMEMcontaining 10%FBS,while the cells in the experimental group were treated with Lipofectamine2000 and PHB siRNA.After incubation for 24 hours,RT-PCR and Western blotting were used to detect the mRNA and protein expression of PHB in transfected A375 cells.Spreading experi-ment was performed for assessing the cell spreading at 30,60 and 120 min.The migration and invasion abilities of A375 cells were detected by Scratch and Transwell invasion assay,respectively.Results The mRNA and protein expression lev-els of PHB in the experimental group were lower than those in the control group (all P<0.01).Compared with the control group,the spreading cells in the experimental group were less at 30,60 and 120 min (all P<0.01).The migration area in the experimental group was significantly less than that in the control group at 12 and 24 h after wounding healing (P<0.01).Meanwhile,the invasive ability of the experimental group was less than that of the control group (P<0.01).Con-clusion PHB gene silencing can effectively inhibit the spreading,migration and invasion of melanoma cells.
Keywords：prohibitinmelanomaspreadingmigrationinvasionRNA interference
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 27-30 )