Effects and mechanisms of inflammation on the calcification of rat vascular smooth muscle cells caused by high phosphate
FENG Ling
ZHANG Yi
JIANG Huiling
FENG Zhiqiang
PAN Qiangwen
Abstract：Objective To study the effects and possible mechanisms of inflammation on the calcification of rat vascu-lar smooth muscle cells (VSMCs)primarily caused by high phosphate.Methods Rat VSMCs were primarily cultured and then were divided into the control (Ctr)group,high phosphate (Pi)group,inflammation (LPS)group,high phosphate and inflammation (Pi+LPS)group.Rat VSMCs in the Pi group and Pi+LPS group were cultured in basal medium contai-ning 4.8 mmol/L Pi to establish the calcification models successfully.Rat VSMCs in the Pi +LPS group were cultured in basal medium containing 4.8 mmol/L Pi and 10 μg/mL LPS.Rat VSMCs in the LPS group were cultured in basal medium only containing 10 μg/mL LPS.Rat VSMCs in the Ctr group were cultured in basal medium.The C-reactive protein (CRP)level of culture medium,alkaline phosphatase (ALP)activity and calcium deposition of cells in each group were respectively measured after 48-hour culture;real-time PCR was used to detect the relative mRNA expression of bone mor-phogenetic protein-2 (BMP-2),Smad4,Msx2 and Osterix.Results The CRP level of culture medium,ALP activity and calcium deposition of cells in the Pi group,LPS group and Pi+LPS group were higher than those of the Ctr group (all P<0.05).The CRP level of culture medium,ALP activity and calcium deposition of cells in the Pi+LPS group were higher than those in the Pi group and LPS group (all P<0.05).The CRP level of culture medium and calcium deposition of cells in the LPS group were lower than those of the Pi group (all P<0.05).The relative mRNA expression of BMP-2,smad4, msx2,and Osterix in the Pi group,LPS group and Pi+LPS group was higher than that of the Ctr group (all P<0.05 ). The relative mRNA expression of BMP-2,smad4,msx2 and Osterix in the Pi+LPS group was higher than that of the Pi group amd LPS group (all P<0.05 ).The relative mRNA expression of smad4,msx2 and Osterix in the LPS group was lower than that of the Pi group (all P<0.05).Conclusions The calcification of rat VSMCs cultured in vitro could be in-duced by both high phosphate and inflammation.Inflammation combined with high phosphate could aggravate the calcifica-tion of rat VSMCs cultured in vitro significantly.The mechanisms may be ralted with the up-regulation of BMP-2,Smad4, Msx2,Osterix expression and the ALP activity.
Keywords：inflammationhigh phosphatesmooth muscle cellsvascular calcificationend-stage renal disease
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 15-19 )