Effects of PI3K/Akt signaling pathway on acetaldehyde-stimulated proliferation of hepatic stellate cells HSC-T6
ZHANG Di
CHEN Min
KANG Xindan
CHEN Weigang
ZHENG Yong
Abstract：Objective To investigate the effects of phosphatidylinositol 3-kinase (PI3K)/Akt signaling pathway spe-cific inhibitor LY294002 on the proliferation of rat hepatic stellate cells (HSC-T6)stimulated by acetaldehyde.Methods The rat HSC-T6 cells were randomly divided into six groups:the blank control group (group A):we used DMEMculture solution with 10% fetal bovine serum (FBS)to culture,the acetaldehyde group (group B):we added acetaldehyde on the basis of the blank control group and made the final concentration be 200 μmol/L,the experimental group was added with different concentrations of PI3K/Akt pathway specific inhibitor LY294002 (25,50,75 and 100 μmol/L)on the basis of acetaldehyde group (group B),and then they were named groups C,D,E and F,respectively.CCK-8 was applied to de-tect the proliferation activity of rat HSC-T6 cells.And then HSC-T6 cells were randomly divided into three groups:the blank control group (added with DMEMculture solution with 10% fetal bovine serum),acetaldehyde group (added with acetaldehyde on the basis of blank control group and made the final concentration be 200 μmol/L),and acetaldehyde +LY294002 group (added with 50 μmol/L PI3K/Akt pathway specific inhibitor LY294002 on the basis of acetaldehyde group).Western blotting was used to detect the expression of PI3K and p-Akt protein in rat HSC-T6.Results The A val-ues of groups A,B,C,D,E,and F were 0.545 0 ±0.024 4,0.952 3 ±0.045 5,0.927 3 ±0.041 9,0.865 8 ±0.028 9,0.814 3 ±0.024 7 and 0.759 3 ±0.220 0,respectively.Compared with group A ,the A values of other groups all in-creased;compared with groups B and C,the A values of groups D,E and F all decreased (all P <0.01).Compared with the blank control group,the protein expression of PI3K and p-Akt in acetaldehyde group and acetaldehyde +LY294002 group were increased (all P <0.01).Compared with the acetaldehyde group,the value of acetaldehyde +LY294002 group decreased (P <0.05).Conclusion PI3K/Akt signaling pathway may partly adjust and control the proliferation of rat HSC-T6 stimulated by acetaldehyde.
Keywords：hepatic fibrosishepatic stellate cellacetaldehydephosphatidylinositol 3-kinase (PI3K)/Akt signaling pathwayLY294002
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 6-8 )