Genotyping of Pseudomonas aeruginosa clinical isolates

ZHOU Xiaoqing; XIE Yantong; LIANG Hongjie; WANG Ke; LI Yanan; KONG Jinliang

doi:10.3969/j.issn.1002-266X.2016.44.005

Abstract：Objective To analyze the genotypes of Pseudomonas aeruginosa ( PA) isolated from inpatients with long-term and continuous infection in various departments of hospital , the and to explore the genetic variation of PA .Methods The strains of PA were collected from specimens of various departments of inpatients , and then we amplified the strains in which the infection lasted for more than 30 days, obtained the RAPD fingerprinting by random amplified polymorphic DNA ( RAPD) genotyping technique , analyzed homology by homology clustering , and understood the genetic relationship be-tween PA through the similarity coefficient .At the same time, we compared the RAPD type to determine whether there was an epidemic outbreak and whether there was a variation between the strains from the same patients with continuous infec -tion.Results A total of 293 strains of PA were collected , including 117 strains in which the infection duration was longer than 30 days, and then 19 genotypes were amplified with A-S type, and the classification rate is 100%.The similarity co-efficient ranged from 0.64 to 0.93, the similarity coefficient of genotypes A and G , genotypes B and R , genotypes D and E, genotypes M and P was 0.93, indicating a high degree of homology between them .In the intensive care unit and burn plastic surgery , the same RAPD type was detected in different patients , the rest of the patients had different RAPD types . The PA fingerprints of 4 patients who suffered continuous infection changed .Conclusion There are many genotypes in PA isolates from patients in various departments of the hospital , which suggests that PA gene mutation occurs in the process of infection, and there is a genetic polymorphism .