Effects of DianhydrogaIactitoI on ceII proIiferation and apoptosis of human gIioma BT325 ceIIs and aIternation in mitochondriaI membrane potentiaI

JIANG Xia; HUANG Yingmei; WANG Xiaojie; LIU Huagang

doi:10.3969/j.issn.1002-266X.2016.43.001

Abstract：Objective To investigative the effects of dianhydrogalacitol(DAG)on cell proliferation and apoptosis of human glioma BT325 cells and its potential mechanism.Methods BT325 cells were divided into the control group and DAG-treated groups at various oncentrations.CCK-8 assay and colony formation assay were performed to detect the cell via-bility of BT325 cells.Hoechst33342 was employed to observe nucleus morphological changes and apoptotic rate.The mito-chondrial membrane potential (MMP)was detected by using Rhodamine 123 staining.Results With the increasing DAG concentration,the survival rate of BT325 cells decreased gradually (P <0.05).The survival rate of BT325 cells decreased with the prolonged incubation time in DAG-treated groups (all P <0.05).The IC50 of BT325 cells treated by DAG were 108.81,26.61,and 11.38 μg/mL at 24,48,and 72 h,respectively.The result of colony formation assay showed that DAG inhibited clones of BT325 cells and the colony formation rate in the control group was 16.78% ±1.50% (all P <0.01).The apoptotic rates of DAG-treated groups treated with 5,10,20,40 and 80 μg/mL DAG were 11.79% ± 2.91%,15.42% ±3.06%,38.70% ±1.12%,60.78% ±1.30%,and 80.35% ±3.22%,respectively,which were in an dose-dependent manner and were significantly increased as compared with that (4.27% ±1.72%)of the control group (all P <0.01).The MMP of control group and DAG-treated groups(5,10,20,and 40 μg/mL)were 0.193 ±0.014, 0.174 ±0.038,0.142 ±0.037,0.138 ±0.033,and 0.119 ±0.013,respectively.Significant difference was found in the MMP between the DAG-treated groups (20 and 40 μg/mL)and the control group (P <0.05 and P <0.01).Conclusions DAG inhibits the proliferation and induces apoptosis of BT325 cells.It is suggested that decreased MMP levels might in-volve in the mechanism.