Effect of MEBT/MEBO on expression of TNF-αand IL-6 in tissue homogenate of chronic non-healing skin wound in rats
MO Xiaoqiang
JIN Meng
TANG Qianli
WU Biaoliang
HUANG Xin
HE Xiaowei
LI Hui
ZENG Hongmeng
WANG Shu
Abstract：Objective To investigate the effects mechanism of skin regeneration medical technique (Moist expose burn therapy/Moist expose burn ointment,MEBT/MEBO)on TNF-αand IL-6 in the granulation tissues in the chronic non-healing skin wounds of rats.Methods Fifty-four SD rats were randomly divided into the observation group,rb-BFGF group and model group.After making the successful models of skin ulcer,the rats in the above groups were respectively posted with two layers of MEBO gauze,bFGF impregnated gauze,and saline gauze.In the blank group,we established models with full-thickness skin defect,without any intervention.Five rats were randomly chosen and killed separately 3,7 and 14 days in every group after the modeling.The granulation tissues in the same part of the skin ulcer wound were ob-tained and the histological structures were detected by HE staining.TNF-αand IL-6 expression was examined by ELISA technique.Results A large amount of micro angiogenesis generated in rats of the observation group and rb-BFGF group 14 days after the treatment.Besides,the inflammation cells disappeared and the skin wounds recovered well.The content of TNF-αand IL-6 was gradually decreased in all groups,the decline in the observation group was the most obvious.During the modeling,the expression of TNF-αand IL-6 in the observation group and rb-BFGF group was always higher than that in the blank group and the difference was statistically significant (all P <0.01).Seven days after modeling,the expression of TNF-αand IL-6 in the observation group was lower than that in the rb-BFGF group and the difference is statistically signifi-cant (P <0.01).Conclusion MEBT/MEBO could control the release of TNF-αand IL-6,change the pathological state of inflammation so as to promote the wound healing.
Keywords：chronic non-healing skin woundskin regeneration medical techniquetumor necrosis factor-αinterleu-kin-6
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 24-27 )