Effects of formula of Herba Erigerontis and Radix Paeoniae Rubra in OGD/R on oxidative stress and NF-κB signaling pathway of nerve cells after ischemia-reperfusion injury
DONG Yongxi
WANG Xia
DONG Li
LIU Zongyan
WANG Aimin
LAN Yanyu
Abstract：Objective To observe the effect of the formula of Herba Erigerontis and Radix Paeoniae Rubra in oxygen-glucose deprivation/reoxygenation (OGD/R)on oxidative stress and nuclear factor-κB (NF-κB)signaling pathway of nerve cells (PC12 cells)after ischemia-reperfusion injury and to investigate its mechanism of protective effect.Methods The PC12 cells were cultured and divided into the control group,model group and low-dose,medium-dose and high-dose formu-la groups.The models of ischemia-reperfusion injury in the model group and formula groups were established by OGD/R. Then the rats in the low-dose,medium-dose and high-dose formula groups were treated with 0.31,0.62,and 1.25 mg/L formula of Herba Erigerontis and Radix Paeoniae Rubra,and all were intervened for 4 h.The control and model groups were treated with DMEMonly.The activity of superoxide dismutase (SOD)and the level of malondialdehyde (MDA)in cell supernatant were detected by ELISA.The protein expression of NO synthase (iNOS),cyclooxygenase-2 (COX-2)and the related proteins (IκBα,p-IκBαand p65)of NF-κB signaling pathway was examined by Western blotting.Results Compared with the control group,the level of SOD was decreased,the level of MDA was increased in the model group (all P <0.01).Compared with the model group,the level of SOD was increased in the medium-dose and high-dose formula groups (P <0.05 or P <0.01).The level of MDA was decreased in all formula groups (P <0.05 or P <0.01).Com-pared with the control group,the protein expression of iNOS and COX-2 was decreased in the model group (all P <0.01). Compared with the model group,the protein expression of iNOS and COX-2 was decreased in all formula groups (P <0.05 or P <0.01).Compared with the control group,the value of p-IκBα/IκBαin cytoplasm,and p65 in nucleus of the model group was increased (all P <0.01).Compared with the model group,the value of p-IκBα/IκBαin cytoplasm and p65 in nucleus of all formula groups (P <0.05 or P <0.01).Conclusions The formula has protective effect on PC12 cells after ischemia-reperfusion injury.The mechanism may be related to the reduction of intracellular oxidative stress response and inhibition of NF-κB pathway activation.
Keywords：ischemia-reperfusion injuryneural cellsHerba Erigerontis and Radix Paeoniae RubraChinese medi-cineoxidative stressnuclear factor-κBinflammatory responsecerebral ischemia
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 5-8 )