Protective effect of BIumea megacephaIa on acute Iiver injury in mice

LIU Guibiao; ZHANG Mingfeng; SHEN Lu; HUANG Jianqiu; GUO Ying; HUANG Wenfei; BI Lihua; WANG Baoshan; WANG Caibing; FANG Xiaoyan

doi:10.3969/j.issn.1002-266X.2016.41.004

Abstract：Objective To investigate the protective effect of Blumea megacephala extracting solution on acute liver injury of mice and its mechanism of action.Methods Fifty KMmice were randomly divided into 5 groups.Normal con-trol group and model control group were given 0.9% NaCl injection,bifendatatum group was given 0.2 g/kg bifendatatum, Blumea megacephala low-concentration group and high-concentration group were respectively given 15 and 30 g/kg Blumea megacephala extracting solution for 7 days by gastric irrigation.Meanwhile,on day 1,3 and 5 of gastric irrigation,the nor-mal control group was given 0.9% NaCl by intraperitoneal injection,the rest of the groups were given 0.1%Carbon tetra-chloride (CCL4 )liquid.One hour 1 h after the last intragastric administration,we collected and separated the serum,and took the left lobe of liver to make and separate the liver supernatant,took the right lobe of liver to make tissue section.The total protein (TP),glutamic-pyruvic transaminase (GPT),glutamic-oxalacetic transaminase (GOT),superoxide dis-mutase (SOD)and malondialdehyde (MDA)content or activity in liver homogenate and serum were detected and we ob-served the structure changes of liver tissue microscopically.Results The TP content of liver homogenate in the model con-trol group,bifendatatum group and Blumea megacephala low-concentration group was obviously higher than that of the nor-mal control group (all P <0.01).The TP content of liver homogenate in the Blumea megacephala high-concentration group was higher than that of the normal control group and model control group (all P <0.05).The GPT activity of liver homoge-nate in the normal control group,model control group and Blumea megacephala low-concentration group was higher than that of the bifendatatum group and Blumea megacephala high-concentration group (all P <0.05).The GOT activity of ser-um in the Blumea megacephala low-concentration group was lower than that of the normal control group (P <0.05).The SOD activity of liver homogenate in the Blumea megacephala high-concentration group,bifendatatum group and model con-trol group was lower than that of the normal control group (all P <0.05).The SOD activity of liver homogenate in the Blumea megacephala low-concentration group was higher than that of the bifendatatum group (P <0.05),but the SOD ac-tivity of serum was higher than that of the normal control group,model control group,bifendatatum group and Blumea megacephala high-concentration group (P <0.01).The MDA content of liver homogenate in the Blumea megacephala low-concentration group and high-concentration group was lower than that of the normal control group (all P <0.05).No statis-tically significant difference were found in the TP content,GPT activity,GOT activity,SOD activity and MDA content of serum among these groups (P >0.05).The histological examinations showed that the liver tissue structure was clear,less liver cells were degenerated,dissolved and necrotic and a small number of central venous cavities had a small amount of shedding liver tissue structure in the bifendatatum group,Blumea megacephala low-concentration and high-concentration group as compared with the model control group.Conclusions Blumea megacephala have protective effect on CCl4-in-duced acute liver injury with dose-effect relationship.The high-concentration Blumea megacephala have the same effect as bifendatatum on liver cells,whose mechanism may be related to facilitation and regulation of SOD in scavenging free radi-cals,blocking and reducing lipid peroxidation of liver cells,and enhancing protein synthesis in liver.