Effects of 1,25(OH)2 D3 on proliferation and cell cycle of human glomerular mesangial cells
NIE Qingqing
TANG Yuling
ZHAO Dan
ZHANG Chunjiang
YANG Xiaoping
Abstract：Objective To investigate the effects of 1 ,25-dihydroxyvitamin D3 [1 ,25 (OH)2 D3 ]on cell proliferation and cell cycle of human glomerular mesangial cells and the possible mechanism.Methods Human mesangial cells in the logarithmic phase were cultured in vitro and then were divided into four groups:groups A,B,C and D.Four groups were separately added with such culture mediums:group A with 10 -8 mol/L 1,25(OH)2D3 +L-DMEMmedium containing 5%fetal bovine serum (FBS),group B with 5 μg/mL rapamycin +L-DMEM medium containing 5% FBS,group C with 5μg/mL rapamycin +10 -8 mol/L 1,25(OH)2D3 +L-DMEMmedium containing 5%FBS,and group D with the L-DMEM medium containing 5% FBS.After 48-hour administration,we observed the cell proliferation and cell cycle distribution in each group.The protein expression of eukaryotic cell translation initiation inhibitory factor 4E binding protein (4E-BP1 ) and phosphorylated 4E-BP1 (P-4EBP1 )in cells was determined by Western blotting.Results At 48 h,the cell apoptosis rates of the groups A,B and C were 38.02%,55.62% and 99.23%,respectively,and significant difference was found between them (P<0.05).At 48 h,the proportion of cells in the G1 phase of groups A,B and C was significantly higher than that of group D (all P<0.05),the proportion of cells in the S,G2/Mphase was clearly lower than that of group D (all P<0.05).The proportion of cells in the G1 phase of groups B and C was significantly higher than that of group A (all P<0.05),and the proportion of cells in the S,G2/Mphase was clearly lower than that of group D (all P<0.05).The proportion of cells in the G1 phase of group C was higher than that of group B (all P<0.05),and the proportion of cells in the S,G2/Mphase was lower than that of group B (all P<0.05).The 4E-BP1 and P-4E-BP1 protein expression and P-4E-BP1/4E-BP1 of groups A,B and C was lower than that of group D (all P<0.05).The 4E-BP1 and P-4E-BP1 protein expression and P-4E-BP1/4E-BP1 of groups B and C was lower than that of group A (all P<0.05).The 4E-BP1 and P-4E-BP1 protein expression and P-4E-BP1/4E-BP1 of group C was lower than that of group B (all P<0.05).Conclusion 1 ,25 (OH)2 D3 may inhibit the glomerular mesangial cell proliferation and block the cell cycle in G1 phase through down-regulating the expression of 4E-BP1 .
Keywords：human glomerular mesangial cell line125-dihydroxyvitamin D3eukaryotic cell translation initiation inhibitory factor 4E binding proteincell proliferationcell cycle
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 12-15 )