Expression of UHRF1 gene in gastric cancer resistant cells and its relationship with multidrug resistance
ZHOU Lin
LI Wei
HAN Dongsheng
ZHANG Wei
Abstract：Objective To observe the expression changes of ubiquitin-like with PHD and ring finger domain 1 (UHRF1 )in gastric cancer drug-resistance cells,and to explore the relationship with gastric cancer multidrug resistance. Methods We examined UHRF1 mRNA and protein expression in GC multidrug-resistant cell lines SGC7901/VCR and SGC7901/ADR and their parental cell line SGC7901 by RT-PCR and Western blotting.Small interfering RNA (siUHRF1) was transfected into SGC7901/VCR and SGC7901/ADR cells (SGC7901/VCR-siUHRF1,SGC7901/ADR-siUHRF1),re-spectively;and blank control group without any treatment was set.UHRF1 plasmid with overexpression (SGC7901-UHRF1)and empty blank plasmid (SGC7901-NC)were transfected into SGC7901 cells,and blank control group was also established.After 48-hour transfection,the cells were collected for further use.MTT assay was conducted to detect the GC cell line sensitivity to several chemotherapy drugs,including VCR,ADR,5-FU and CDDP,and IC50 was calculated.5-FU was co-cultured with gastric cancer cell lines and the apoptosis was observed by flow cytometry (FCM).Results The rela-tive mRNA and protein expression of UHRF1 in SGC7901 cell line was down-regulated as compared with that of SGC7901/VCR and SGC7901/ADR cells (all P<0.05 ).Promoting the expression of UHRF1 could increase the IC50 of SGC7901 cells to VCR,ADR,5-FU and CDDP and the apoptosis was decreased (all P<0.05).Silencing UHRF1 expression could reduce the IC50 of SGC7901/VCR and SGC7901/ADR cells to VCR,ADR,5-FU and CDDP,and the apoptosis rate was increased (all P<0.05).Conclusions UHRF1 is highly expressed in gastric cancer drug-resistance cell lines,and the high expression of UHRF1 may decrease drug sensitivity and improve multidrug resistance of gastric cancer,which may be related to the inhibition of UHRF1 on the apoptosis of gastric cancer cells.
Keywords：gastric carcinomaubiquitin-like with PHD and ring finger domain 1multidrug resistancedrug sensi-tivitymedian inhibitory concentrationapoptosis
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1-4 )