Observation of cell proliferation and differentiation in rat osteoblasts tranfected by ClC-3 siRNA
LI Qin
HE Lingli
CHEN Tingting
LONG Mimi
WANG Cheng
Abstract：Objective To observe the cell proliferation and differentiation in rat osteoblasts which were transfected by ClC-3 siRNA.Methods The primary osteoblasts were extracted and divided into 4 groups:the control group which was not treated, negative control ( NC) group which was transfected by unordered siRNA, Lipo group which was transfected by li-pofectamineTM2000 transfection reagents only and the ClC-3 siRNA group which was transfected by ClC-3 siRNA.All cells were cultured in the flow shear stress installation.The optical density (OD) values at 24, 48 and 72 h after culture in each group were detected by MTT.The alkaline phosphatase level and calcified nodule was also detected.Results Compared with the control group, the OD value of the ClC-3 siRNA group was decreased at each time points (all P<0.05).The al-kaline phosphatase levels and calcified nodule forming abilities in the ClC-3 siRNA, Lipo, NC and control groups were (32.434 ±2.130), (46.199 ±1.900), (47.347 ±2.500) and (49.540 ±1.550) U/mL, and 25.02%±3.46%, 36.83%±4.54%, 38.45%±4.84%and 40.57%±2.22%, respectively.Significant difference was found between the ClC-3 siRNA group and the control group (all P<0.01).Conclusion After transfection of ClC-3 siRNA, the cell prolif-eration and differentiation of rat osteoblasts is inhibited.
Keywords：gene transfectionClC-3 proteinosteoblastschloride channelflow shear stresscell proliferationcell differentiationalkaline phosphatase
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 24-27 )