Effects of curcumol combined with oxaliplatin on xenograft growth and proliferation of gastric cancer tumor cells in nude mice
WANG Dong
LI Yong
GUO Zhipeng
ZHAO Qun
FAN Liqiao
TAN Bibo
CUI Ping
Abstract：Objective To observe the effects of curcumol combined with oxaliplatin on xenograft growth of gastric cancer cells in nude mice, then to explore the mechanism.Methods Twenty nude mice were selected to establish the xen-ograft models of gastric cancer cells in nude mice.Nude-mice xenograft models were randomly divided into four groups ( n=5 in each group): the control group, curcumol group, oxaliplatin group and curcumol combined with oxaliplatin group (combined treatment group), then they were respectively treated with normal saline (0.05 mL/10 g), curcumol (100 mg/kg), oxaliplatin (30 mg/kg) and curcumol (100 mg/kg) combined with oxaliplatin (30 mg/kg), once every 4 days for four times.The growth of tumor was recorded.The cell survival rate of tumor cells in cancer tissues was determined by MTT.The expression levels of Caspase-3, Bcl-2 mRNA and protein were detected by fluorescence quantitative RT-PCR and immunohistochemistry.Results Compared with the control group, the tumor volume, weight, cell survival rate and the expression of Bcl-2 was decreased but the expression of Caspase-3 was increased in the curcumol group, oxaliplatin group and combined treatment group, and these changes were more significant in the combined treatment group (all P<0.05). Conclusion The curcumol combined with oxaliplatin may inhibit the xenograft growth and proliferation of gastric cancer tumor cells in nude mice, and the mechanism may be related to the up-regulation of Caspase-3 and down-regulation of Bcl-2.
Keywords：curcumoloxaliplatingastric carcinomaxenograft models in nude miceBcl-2Caspase-3
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 21-23 )