Sensitivity change of ovarian cancer cells transfected with miR-101 analogues to paclitaxel
HU Keke
LIU Xiaomin
SONG Hong
YU Yuanyi
Abstract：Objective To observe the sensitivity change of ovarian cancer cells transfected with miR-101 analogues to paclitaxel, and to explore its mechanism.Methods Ovarian cancer cells SKOV3 were cultivated and divided into 3 groups:the observation group, control group and single drug group.Three groups were treated with 0, 6.25, 25, 100, 400 and 1 600 ng/mL paclitaxel.The observation group was transfected with miR-101 mimics, control group was transfected with unrelated sequence and single drug group was not transfected with any sequence.MTT was conducted to observe the proliferation ability of cells in each group.Annexin V/propidium iodide staining was employed to detect the apoptosis rate in each group.Western blotting was employed to detect the protein expression of DNMT3A in these three groups which were respectively treated by 25 ng/mL paclitaxel.Results The OD values of the observation group were 0.632 ±0.014, 0.554 ±0.012, 0.503 ±0.015, 0.428 ±0.011, 0.346 ±0.006 and 0.301 ±0.007, respectively.The OD values of the single drug group were 0.718 ±0.013, 0.687 ±0.015, 0.619 ±0.011, 0.554 ±0.009, 0.473 ±0.008 and 0.420 ±0.011, respectively.The OD values of the control group were 0.713 ±0.016, 0.671 ±0.011, 0.626 ±0.010, 0.549 ±0.008, 0.467 ±0.010 and 0.412 ±0.008, respectively.The differences were of statistical significance between the observation group and control group (all P<0.05).The apoptosis rates of the observation group were 9.63%±0.38%, 14.71%± 0.64%, 21.27%±0.79%, 27.05%±0.92%, 31.82%±1.07%, 35.51%±1.63%, respectively.The apoptosis rates of the single drug group were 3.61%±0.24%, 6.18%±0.48%, 9.57%±0.42%, 15.64%±0.75%, 18.79%±1.12%and 22.05%±1.34%, respectively.The apoptosis rates of the control group was 3.74%±0.29%, 6.02%± 0.35%, 10.63%±0.58%, 15.21 %±0.84%, 19.37%±1.26% and 23.30%±1.18%, respectively.The differ-ences were of statistical significance between the observation group and control group (all P<0.05).The expression of DNMT3A protein in the observation group, control group and single drug group were 0.427 ±0.068, 0.961 ±0.107 and 0.947 ±0.113, respectively.The differences were of statistical significance among these three groups (P<0.05).Con-clusion MiR-101 analog can increase the sensitivity of ovarian cancer cells to paclitaxel through targeted-regulating DN-MT3A.
Keywords：miR-101 analoguesovarian carcinoma cellsmethylation transferase 3Apaclitaxeldrug susceptibility
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 13-16 )